Marvin W Makinen
Research Summary / Selected Publications
Our research is directed towards the study of the structural basis of enzyme action through a detailed correlation of the electronic structure, stereochemistry, and molecular structure of enzyme-substrate complexes. In these studies we have developed methods to stabilize catalytically active intermediates of enzyme reactions with use of organic-aqueous cosolvent mixtures at sub-zero temperatures for structural characterization by magnetic resonance methods. This information provides a picture of the structural basis of interactions between the substrate and enzyme that lead to catalysis. To further understand the structural basis of enzyme action, the interactions of the substrate with active site residues structurally defined through measurement of critical interatomic distances using magnetic resonance methods are analyzed with use of computer controlled molecular graphics and correlated with results of kinetic, chemical, and mutagenic experiments.
Our investigations have been conducted through the application of electron spin resonance (ESR) and electron nuclear double resonance (ENDOR) spectroscopy. We have shown that the accuracy of structural information obtained with ENDOR is exceeded only by that of single crystal X-ray...
Mustafi, D., Peng, B., Makinen, M. W., Karczmar, G. S., Zamora, M., Ejnik, J., and Martin, H. (2009). "New Vanadium-based Magnetic Resonance Imaging Probes: Clinical Potential for Early Detection of Cancer." J. Biol. Inorg. Chem. 14: 1187-1197.
Mustafi, D., and Makinen, M. W. (2005). "Structure and conformation of bis(acetylacetonato)oxovanadium(IV) and bis(maltolato)oxovanadium(IV) in solution determined by electron nuclear double resonance spectroscopy." Inorg. Chem. 44:5580-5590.
Ou,H., Yan, L., Mustafi, D., Makinen, M. W., and Brady, M. J. (2005). "The vanadyl (VO2+) chelate bis(acetylacetonato)oxovanadium(IV) potentiates tyrosine phosphorylation of the insulin receptor." J. Biol. Inorg. Chem. 10: 874-886.
Mustafi, D. & Makinen, M. W. (2004). Application of Angle-Selected Electron Nuclear Double Resonance to Characterize Structured Solvent in Small Molecules and Macromolecules. In Biological Magnetic Resonance. (G. A. Eaton, S. Eaton & L. J. Berliner, Eds.) 24: 89-144, Plenum Press, New York.