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Shohei Koide

Professor, Biochemistry & Molecular Biophysics, Committee on Cancer Biology


B.Sc. University of Tokyo, 1986; Ph.D. University of Tokyo, 1991; Postdoctoral, The Scripps Research Institute

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900 E. 57th Street
Chicago, IL 60637
KCBD 3250
Phone: (773) 702-5073
Fax: (773) 702-0439

900 E. 57th Street
Chicago, IL 60637
KCBD 3230
Phone: (773) 702-5513

Shohei Koide

Research Summary / Selected Publications

We are interested in creating proteins with novel functions and using such designer proteins to control biology. Our research is interdisciplinary, integrating approaches in directed evolution, structural biology, protein chemistry, spectroscopy and cell biology.

Protein Design. We have taken a "minimalist" approach for understanding the determinants for tight and specific interfaces (e.g. how much chemical and structural diversity is required for affinity and specificity?) by constructing small but highly functional binding proteins. This exercise has substantially advanced our understanding of the molecular mechanisms underlying molecular recognition and protein evolution.

We use two platforms. The first is the "monobodies", a small protein that presents surface binding loops, and "synthetic antibodies", both representing a ubiquitous mode of binding seen in natural proteins. The second is the "affinity clamps", a two-domain architecture that we have created specifically for recognizing flexible peptide segments. We combine structure-guided design and directed evolution to efficiently generate synthetic binding proteins.

Controlling signaling and epigenetic networks...


Yasui N, Findlay GM, Gish GD, Hsiung MS, Huang J, Tucholska M, Taylor L, Smith L, Boldridge WC, Koide A, Pawson T, Koide S. (2014) Directed Network Wiring Identifies a Key Protein Interaction in Embryonic Stem Cell Differentiation. Mol Cell. 54:1034-41.  PubMed

Hattori T, Taft JM, Swist KM, Luo H, Witt H, Slattery M, Koide A, Ruthenburg AJ, Krajewski K, Strahl BD, White KP, Farnham PJ, Zhao Y, Koide S. (2013) Recombinant antibodies to histone post-translational modifications. Nat Methods 10:992-5.  PubMed

Sha F, Gencer EB, Georgeon S, Koide A, Yasui N, Koide S, Hantschel O. (2013) Dissection of the BCR-ABL signaling network using highly specific monobody inhibitors to the SHP2 SH2 domains. Proc Natl Acad Sci U S A. 2013 Aug 26.   PubMed

Gibreth RN, Koide S. (2012) Structural Insights for Engineering Binding Proteins Based on Non-Antibody Scaffolds. Curr Opin Struct Biol, 22:413-20.  PubMed

Koide A, Wojcik J, Gilbreth RN, Hoey RJ, Koide S. (2012) Teaching an Old Scaffold New Tricks: Monobodies Constructed Using Alternative Surfaces of the FN3 Scaffold. J Mol Biol. 415:393-405.  PubMed