Geoffrey L Greene
Research Summary / Selected Publications
The overall goal of my research is to determine the molecular mechanisms by which female steroid hormones regulate development, differentiation and/or cellular proliferation and survival in hormone responsive tissues and cancers. Estrogens regulate the expression of diverse regulatory proteins and growth factors via one or both of two estrogen receptor subtypes (ERα & ERβ). My lab is actively studying several aspects of ER action, including the role of post translational modifications in the transcriptional activation of ER, the roles of ER-associated proteins in receptor-mediated responses, the molecular nature of transcriptional activation and/or repression in the regulation of target gene expression, nongenomic actions of estrogens, and the detailed structural requirements for ligand binding to ERα/β, especially in regard to discrimination between estrogen agonists and selective estrogen receptor modulators (SERMs). I am also interested in using high throughput sequencing to identify and characterize ERα/β genomic targets and their regulated transcripts in breast cancer cells, as well as the transcription factors that co-associate at these targets and the mechanisms by which distal DNA response elements elements are able...
Sinkevicius, K.W., Laine, M., Lotan, T.L., Woloszyn, K., Richburg, J.H., and Greene, G.L. (2009). Estrogen-dependent and -independent estrogen receptor-alpha signaling separately regulate male fertility. Endocrinology 150, 2898-2905.
Nettles, K.W., Gil, G., Nowak, J., Metivier, R., Sharma, V.B., and Greene, G.L. (2008). CBP Is a dosage-dependent regulator of nuclear factor-kappaB suppression by the estrogen receptor. Molecular endocrinology (Baltimore, Md 22, 263-272.
Hsieh, R.W., Rajan, S.S., Sharma, S.K., and Greene, G.L. (2008). Molecular characterization of a B-ring unsaturated estrogen: Implications for conjugated equine estrogen components of Premarin. Steroids 73, 59-68.